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Radiotoxicity of11C-methionine measured by the accumulation of DNA strand breaks in mammalian cells

✍ Scribed by Carl-Göran Stålnacke; Synnöve Sundell-Bergman; Christer Halldin; Bengt Långström

Publisher
Springer
Year
1985
Tongue
English
Weight
535 KB
Volume
11
Category
Article
ISSN
0340-6997

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✦ Synopsis

The radiotoxic effects of L-[methyl-11C] methionine were estimated by measuring the accumulation of DNA strand breaks. CHO, Chinese-hamster cells, were incubated in 11C-methionine-containing medium for 60 min at 37 degrees C. The number of unrepaired DNA strand breaks was then examined by the DNA-unwinding method. For comparison, cells were also externally irradiated under similar conditions with gamma radiation (137Cs) or positrons (11C). The relative biological effectiveness of 11C-methionine was estimated to be about 1.

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