Radiosynthesis, biodistribution and scintigraphy of the 99mTc-Teicoplanin complex in artificially infected animal models
✍ Scribed by Syed Qaiser Shah; Aakif Ullah Khan; Muhammad Rafiullah Khan
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- French
- Weight
- 295 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0022-2135
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Radiocomplexation of Teicoplanin (TIN), a new glycopeptide antibiotic with technetium‐99m, was investigated. The ^99m^Tc‐TIN complex was assessed for its radiochemical permanence, in vitro stability in serum, binding with methicillin‐resistant Staphylococcus aureus (MRSA), biodistribution in Model Rats (MRT) and for scintigraphic precision in Model Rabbit (MRB). Radiochemically, a stable ^99m^Tc‐TIN was observed with 98.90±0.50% yield and remained staunch more than 90% up to 120 min, by mixing TIN, 1.5 mg in 0.5 ml of saline with 2.5 mCi sodium pertechnetate and 150 µl of stannous chloride dihydrate at pH 5.4. The ^99m^Tc‐TIN was found stable in serum with an insignificant undesirable yield of free and unhydrolyzed technetium (5.25±0.10 and 13.5±0.14%, n = 10) up to 120 min of incubation. The ^99m^Tc‐TIN showed in vitro binding with MRSA in the range of 55–65%. The ^99m^Tc‐TIN showed almost six‐fold elevated uptake in the infected (IFT) muscle of the MRT as compared with the inflamed (IFM) and normal (NL) muscles. This higher uptake of the ^99m^Tc‐TIN in the IFT was scintigraphically confirmed after the whole body scanning of the MRB. The radiochemical eternalness with high yield, in vitro stability in serum, binding with MRSA, significant biodistribution performance, and scintigraphic precision posed the ^99m^Tc‐TIN, a new glycopeptide radiotracer for the infection imaging. Copyright © 2010 John Wiley & Sons, Ltd.