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Radiometric determination of cytidine 5′-triphosphate in biological materials

✍ Scribed by D.E. Leelavathi; Robert W. Guynn

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
449 KB
Volume
83
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple and sensitive radioenzymic method is described for the direct determination of cytidine 5'-triphosphate (CTP) in the range of 1 to 9 nmol in perchloric acid extracts of tissue. The method involves a specific conversion of CTP and [ 1,2-"C]phosphorylethanolamine to labeled CDP-ethanolamine using purified CTPphosphorylethanolamine cytidylyltransferase (EC 2.7.7.14). The [%Z]CDP-ethanolamine formed is isolated on a Dowex I-formate column taking advantage of the selectivity of borate complexing. A spectrophotometric method for determining CTP in standard solutions is also described.

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