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Radiometric assays for mammalian epoxide hydrolases and glutathione S-transferase

✍ Scribed by Sarjeet S. Gill; Kenji Ota; Bruce D. Hammock


Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
820 KB
Volume
131
Category
Article
ISSN
0003-2697

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A rapid radiometric assay for mammalian
✍ Christopher A. Mullin; Bruce D. Hammock πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 814 KB

A rapid and sensitive radiometric assay for cytosolic epoxide hydrolase is described. The assay is based on the highly efficient partitioning of unreacted I-phenyl-1,2-epoxybutane (P-ethylstyrene oxide) from an aqueous mix into isooctane, with the product diol being retained in the aqueous phase. Th