Radioiodination of the envelope proteins of newcastle disease virus

✍ Scribed by Li, Joseph K.-K. ;Fox, C. Fred

• Book ID: 102925446
• Publisher: Wiley (John Wiley & Sons)
• Year: 1975
• Tongue: English
• Weight: 504 KB
• Volume: 3
• Category: Article
• ISSN: 0091-7419
• DOI: 10.1002/jss.400030106

✦ Synopsis

Abstract

Lactoperoxidase‐catalyzed iodination selectively labels the two glycoproteins (VP1 and VP2) of Newcastle disease virus. The low‐molecular‐weight, nonglycosylated major viral protein, VP6, was not iodinated in the intact virus but was iodinated in disrupted virions, suggesting a localization on the inner, rather than the outer envelope surface. Studies on the distribution of virion proteins labeled with ^125^I and ^3^H‐isoleucine between detergent‐soluble and detergent‐insoluble fractions show that the virion proteins VP4, VP5, and VP6 are solubilized to a much lesser extent than are VP1 and VP2.