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Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent

✍ Scribed by H.J. Sinn; H.H. Schrenk; E.A. Friedrich; D.P. Via; H.A. Dresel

Book ID
102985013
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
539 KB
Volume
170
Category
Article
ISSN
0003-2697

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✦ Synopsis

In an attempt to improve conditions for radioiodination of sensitive proteins we used N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.

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