Radioiodination of a photoactivatable heterobifunctional reagent

✍ Scribed by Inhae Ji; Jaekyoon Shin; Tae H. Ji

• Book ID: 102629476
• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 303 KB
• Volume: 151
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90186-1

✦ Synopsis

The N-hydroxysuccinimide ester of 4azidosalicylic acid, a photoactivable heterobifunctional reagent, can be radioiodinated. The low efficiency (3%) of the radioiodination by a previously published method (1. Ji and T. H. Ji, 1982, Anal. Biochem. 121,286-289) has been increased to 63% by substituting the solvent, acetone, with others such as aqueous acetonitrile, dimethylformamide, or dimethyl sulfoxide. The resulting izJI reagent was used for derivatizing human choriogonadotropin, The radioactive hormone derivative was crosslinked to the @3 dimer upon photolysis.