Radioimmunological determination of cocaine in human hair
โ Scribed by Balabanova, S. ;Brunner, H. ;Nowak, R.
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 330 KB
- Volume
- 98
- Category
- Article
- ISSN
- 1437-1596
No coin nor oath required. For personal study only.
โฆ Synopsis
A simple procedure for the determination of cocaine in human hair was described. After washing hair samples were crushed in 0.1 m HC1 and incubated overnight at 45ยฐC. The acid extracts were neutralized with 1 m NaOH. Phosphate buffer (pH 7.4) was added to the extracts. The cocaine concentrations were measured by radioimmunoassay. Detection in hair was achieved in all hair samples obtained from cocaine users. This method appears to be suitable for the routine determination of cocaine.
Key word: Cocaine, radioimmunological determination in hair
Zusammenfassung. Diese Arbeit beschreibt eine Methode fiir die Bestimmung von Kokain im menschlichen Haar. Das Haar wurde gewaschen, zermalt mit 0,1m HC1 und tiber Nacht bei 45ยฐC inkubiert. Nach Neutralisierung mit 1 m NaOH wurde Phosphat-Puffer (pH 7,4) zugesetzt. Die Kokain-Konzentrationen wurden mittels Radioimmunoassay bestimmt. In allen Haarproben yon Kokainabh~ingigen wurden megbare Konzentrationen nachgewiesen. Dieses Veffahren stellt eine schnelle und einfache Routinemethode dar.
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