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Radioimmunoassay of brain peptides: Evaluation of a methodology for the assay of β-endorphin and enkephalin

✍ Scribed by Jean Rossier; Alejandro Bayon; Therese M. Vargo; Nicholas Ling; Roger Guillemin; Floyd Bloom


Book ID
118938056
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
290 KB
Volume
21
Category
Article
ISSN
0024-3205

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3-Endorphin-like peptides in blood plasma of normal human subjects were studied by means of a radioimmunoassay (RIA) and gel filtration. Plasma was extracted with silica gel, which was washed with water and 1 N HCI, and eluted with 50% acetone. Plasma extracts thus obtained and standard synthetic hu