Radiochemical synthesis and preliminary evaluation of anti-bladder cancer monoclonal antibody BDI-1 labeled with rhenium-188
✍ Scribed by Rongfu Wang; Chungli Zhang; Lizhang Yu; Yifeng Guo; Ying Bai
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- French
- Weight
- 103 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0022-2135
- DOI
- 10.1002/jlcr.470
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✦ Synopsis
Abstract
The anti‐human bladder cancer monoclonal antibody BDI‐1 was radio‐labeled with rhenium‐188 by direct labeling methods using SnCl~2~ as reductant and MDP as stannous stabilizer or by indirect method using NHS‐ECM ester as bifunctional chelator. Radiochemical yields of 30±7.23% and 87.4±5.67% and radiochemical purity of more than 95% were achieved. The inmmunoreactive fraction was 58.7%. The results showed that radionuclide ^188^Re might be employed using the same labeling methodology with ^99m^Tc labeling of BDI‐1 for radiommunoimaging (RII) and radioimmunotherapy (RIT) and that may be useful in radioimmunodetection and RIT of human bladder carcinoma in vivo. Copyright © 2001 John Wiley & Sons, Ltd.