Rabbit eye irritation caused by wearing toxic contact lenses and their cytotoxicities:In vivo/in vitro correlation study using standard reference materials
✍ Scribed by Tsuchiya, Toshie ;Arai, Tadashi ;Ohhashi, Jyunichi ;Imai, Kiyoshi ;Kojima, Hajime ;Miyamoto, Satoru ;Hata, Hideyo ;Ikarashi, Yoshiaki ;Toyoda, Kazuhiro ;Takahashi, Michihito ;Nakamura, Akitada
- Book ID
- 102874192
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 729 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Abstract
To clarify the relationship between eye irritancy and cytotoxic potential induced by irritant materials, we made lenses coated with standard reference materials (SRMs) prepared from various amounts of zinc diethyldithiocar‐bamate (ZDEC) and polyurethane (PU).
Zinc diethyldithiocarbamate was classified as a mild irritant by the Draize eye irritation test. When ZDECSRM coated contact lenses were applied to rabbit eyes, Draize scores increased in proportion to both the ZDEC and PU concentrations used for coating. Furthermore, correlation with the cytotoxic potential (γ = −0.93) was better than with lactate dehydrogenase (LDH) activities of tears from rabbit eyes wearing these coated lenses (γ = 0.78). In conclusion, in vivo eye irritancy induced by wearing lenses could be estimated quantitatively with the cytotoxic potentials using a colony assay. Further‐more, we could compare different sensitivities caused by the same set of SRMs among three different sites of tissue. As a result, the order of sensitivity was eye > muscle ≫ skin. © 1993 John Wiley & Sons, Inc.