Quorum Sensing: Methods and Protocols

✍ Scribed by Livia Leoni, Giordano Rampioni (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 375
• Series: Methods in Molecular Biology 1673
• Edition: 1
• Category: Library

✦ Synopsis

This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Quorum Sensing: Methods and Protocols serves as a comprehensive guide to the most important methodology currently available in the field.

✦ Table of Contents

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
Use of Whole-Cell Bioassays for Screening Quorum Signaling, Quorum Interference, and Biofilm Dispersion (Starla G. Thornhill, Robert J. C. McLean)....Pages 3-24
Detection of 2-Alkyl-4-Quinolones Using Biosensors (Matthew P. Fletcher, Stephen P. Diggle, Miguel Cámara, Paul Williams)....Pages 25-34
“Hot Stuff”: The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals (Amy L. Schaefer, Caroline S. Harwood, E. Peter Greenberg)....Pages 35-47
Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs) (François Lépine, Sylvain Milot, Marie-Christine Groleau, Eric Déziel)....Pages 49-59
Detection of the Bacterial Quorum-Sensing Signaling Molecules N-Acyl-Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS) (Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, Anton Hartmann)....Pages 61-72
Biosensors for the Detection and Quantification of AI-2 Class Quorum-Sensing Compounds (Sathish Rajamani, Richard Sayre)....Pages 73-88
Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus (Ewan J. Murray, Paul Williams)....Pages 89-96
Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF) Family Quorum-Sensing Signals (Lian Zhou, Shuang Sun, Wei Zhang, Ya-Wen He)....Pages 97-105
Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode (Alyah Buzid, John H. T. Luong, F. Jerry Reen, Fergal O’Gara, Jeremy D. Glennon, Gerard P. McGlacken)....Pages 107-116
Detection and Quantification of Butyrolactones from Streptomyces (Marc Biarnes-Carrera, Rainer Breitling, Eriko Takano)....Pages 117-128
Front Matter ....Pages 129-129
Fluorescence Quenching Studies of γ-Butyrolactone-Binding Protein (CprB) from Streptomyces coelicolor A3(2) (Jessy Mariam, Ruchi Anand)....Pages 131-143
Methods to Study Solo/Orphan Quorum-Sensing Receptors (Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, Brian M. M. Ahmer)....Pages 145-159
Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases (Daniel Shin, Rajesh Nagarajan)....Pages 161-176
Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq (Charlotte D. Majerczyk)....Pages 177-192
Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics (Yilei Liu, Gabriella Pessi, Katharina Riedel, Leo Eberl)....Pages 193-202
Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo (Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt)....Pages 203-212
Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells (Jake Everett, Rebecca Gabrilska, Kendra P. Rumbaugh, Elena Vikström)....Pages 213-225
Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies (Damien Maura, Arunava Bandyopadhaya, Laurence G. Rahme)....Pages 227-241
Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta (Shulamit Manulis-Sasson, Laura Chalupowicz)....Pages 243-252
Differential Equations Models to Study Quorum Sensing (Judith Pérez-Velázquez, Burkhard A. Hense)....Pages 253-271
Front Matter ....Pages 273-273
Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro (Tim Holm Jakobsen, Maria Alhede, Louise Dahl Hultqvist, Thomas Bjarnsholt, Michael Givskov)....Pages 275-285
A Coculture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum Sensing Inhibitors (Giordano Rampioni, Giulia Giallonardi, Francesca D’Angelo, Livia Leoni)....Pages 287-296
A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin (Kaihao Tang, Xiao-Hua Zhang)....Pages 297-309
Directed Evolution of Quorum-Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus (Maybelle Kho Go, Jeng Yeong Chow, Wen Shan Yew)....Pages 311-323
Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones (Soumya Palliyil)....Pages 325-352
Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi Through Structure-Based Virtual Screening (Tianyu Jiang, Peng Zhu, Lupei Du, Minyong Li)....Pages 353-362
Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography (Cassandra L. Quave, Alexander R. Horswill)....Pages 363-370
Back Matter ....Pages 371-375

✦ Subjects

Microbiology