Quinacrine and acridine-R banding without a fluorescence microscope

✍ Scribed by Y. Nakagome; S. Oka; M. Higurashi

• Book ID: 104696102
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 578 KB
• Volume: 40
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00272297

✦ Synopsis

A technique is described in which a standard fluorescence microscope equipped with a high-pressure mercury lamp is replaced with an ordinary laboratory microscope fitted with a quartz-iodine lamp. A dark field condenser and a set of three filters, including an FITC interference filter, complete a 'fluorescence' microscope. The microscope has proved itself satisfactory in the study of Y-chromatin, chromosome Q-bands including Q-polymorphism, and acridine-R band. It is very easy to operate and does not emit ultraviolet light, which might harm operators. Total cost of the quartz-iodine lamp's outfit, filters, and a dark-field condenser is much less than that of a standard fluorescence microscope. The cost is especially low when a laboratory microscope with a quartz-iodine lamp is already at hand. Spectrofluorometric studies of QM and Q indicate that the present system will show even better performance if an interference filter with a transmission range of about 400 to 440--450 nm is designed and used in combination with a 455--475 nm barrier filter.