A method for identifying cysteine-containing peptides in proteins is presented using 2-bromoacetamido-4-nitrophenol (BNP) to introduce an easily detectable probe. The formation of a covalent bond between the protein sulfhydryl group and the acetamido moiety of BNP introduces a chromophore with an ab
Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4′-dithiodipyridine
✍ Scribed by Christian K. Riener; Gerald Kada; Hermann J. Gruber
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 136 KB
- Volume
- 373
- Category
- Article
- ISSN
- 1618-2650
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