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Quick assessment of cytotoxins effect on Daphnia magna using in vivo fluorescence microscopy

✍ Scribed by Vera V. Teplova; Zhanna I. Andreeva-Kovalevskaya; Elena V. Sineva; Alexander S. Solonin

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 219 KB
Volume: 29
Category: Article
ISSN: 0730-7268
DOI: 10.1002/etc.169

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✦ Synopsis

Abstract

A novel approach to contaminant toxicity screening is proposed. The use of fluorescent microscopy with fluorescent dyes allows for assessing intoxication of Daphnia magna tissues, at various stages of exposure, to contaminants present in water. As shown, D. magna may not only be used as a test species in toxicity tests based on its lethality, but due to its translucency and application of fluorescent probes, separate steps of its intoxication and dying can be visualized. Using a variety of fluorescent probes, the present study also contributes to a better understanding of the toxicity mechanisms. Environ. Toxicol. Chem. 2010;29:1345–1348. © 2010 SETAC

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