✦ LIBER ✦
Quick and accurate method to convert BCECF fluorescence to pHi: Calibration in three different types of cell preparations
✍ Scribed by Marilyn R. James-Kracke
- Book ID
- 102881369
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 903 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
A rapid, easy, and accurate method for converting the fluorescence of BCECF to pH, as an alternative to the nigericin method, is described. The ratio of the fluorescence intensities for BCECF can be converted to pH between 4 and 9 by a formula similar to the one used to calculate [Ca2'Ii from the fluorescence offuraz.