Quick analysis of enantioselectivity by a chromatographic reactor: II. Experimental verification

✍ Scribed by Jau-Yann Wu; Shi-Wenn Liu

• Publisher: Wiley (John Wiley & Sons)
• Year: 1999
• Tongue: English
• Weight: 90 KB
• Volume: 74
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/(sici)1097-4660(199910)74:10<974::aid-jctb136>3.0.co;2-m

✦ Synopsis

A modi®ed chromatographic process was used to determine the enantiomeric ratio (E) in enantioselective biocatalysis. Theoretical results indicated that the established relationship, E =ln(1Àx S )/ln(1Àx R ), was valid for this modi®ed operation. In addition, the Candida cylindracea lipase-catalyzed enantioselective esteri®cation of racemic naproxen was selected to verify experimentally the effectiveness of the proposed method. The ease of operation and simplicity of data analysis of the proposed method makes it a promising alternative for determining the enantioselectivity of enzymatic chiral resolution.