QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS

✍ Scribed by María Isabel Alarcón Flores; Roberto Romero-González; Antonia Garrido Frenich; José Luis Martínez Vidal

• Book ID: 102444635
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 232 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100093

✦ Synopsis

Abstract

A new method has been developed and validated for the simultaneous analysis of different phytohormones (auxins, cytokinins and gibberellins) in vegetables. The compounds were extracted using a QuEChERS‐based method (acronym of quick, easy, cheap, effective, rugged and safe). The separation and determination of the selected phytohormones were carried out by ultra high‐performance liquid chromatography coupled to tandem mass spectrometry (UHPLC‐MS/MS), using electrospray ionization source (ESI) in positive and negative ion modes. The method was validated and mean recoveries were evaluated at three concentration levels (50, 100 and 250 μg/kg), ranging from 75 to 110% at the three levels assayed. Intra‐ and interday precisions, expressed as relative standard deviations (RSDs), were lower than 20 and 25%, respectively. Limits of quantification (LOQs) were equal or lower than 10 μg/kg. The developed procedure was applied to seven courgette samples, and naphthylacetic acid, naphthylacetamide and benzyladenine were found in some of the analysed samples.