Quasielastic light scattering by biopolymers. V. Interparticle interactions between polynucleosomes
✍ Scribed by Kenneth S. Schmitz; Nambi Parthasarathy; Jacqueline C. Kent; Jennifer Gauntt
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1982
- Tongue
- English
- Weight
- 844 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Quasielastic light scattering and electrophoretic light scattering experiments were performed on chicken erythrocyte polynucleosome solutions at various temperatures and ionic strengths. The apparent diffusion coefficient, D~app~, was found to depend on the scattering vector K. In general, D~app~ can be described as a damped oscillatory function of K in the ionic strength range of 10 to 60 m__M__ and over the temperature range of 10 to 40°C. Electrophoretic light scattering studies on total digest chromatin samples indicate the apparent charge on the polynucleosomes increases as the ionic strength is lowered from 10 to 1 m__M__. These data are interpreted in terms of fluctuations in the surface charge distribution of the polyion and subsequent inducement of an asymmetric distribution of small ions about the polyion. These fluctuation components lead to the formation of “clusters” of polyions.