Quantum-dots-based detection of hepatitis C virus (HCV) NS3 using RNA aptamer on chip

✍ Scribed by Changhyun Roh; Ho-Young Lee; Sang-Eun Kim; Sung-Kee Jo

• Publisher: Wiley (John Wiley & Sons)
• Year: 2010
• Tongue: English
• Weight: 167 KB
• Volume: 85
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.2409

✦ Synopsis

Abstract

BACKGROUND: Over 170 million people, more than 3% of the world's population, suffer from the hepatitis C virus (HCV) infection and the rate of death from liver‐related mortality to HCV has increased. In respect of this, the development of assays for biological imaging should be urgently considered as an essential factor in diagnosis.

RESULTS: A novel HCV‐detecting technique using a nanoparticle‐supported aptamer probe was demonstrated. With the aid of nanoparticle quantum dots (QDs) with carboxyl group as an imaging probe, and 5′‐end‐amine‐modified RNA oligonucleotide as a capturing probe, target HCV NS3 was visually detected on chip. The QDs‐based RNA aptamer for HCV NS3 showed high selectivity and specificity against other protein such as BSA. The detection limit of HCV NS3 protein was 5 ng mL^−1^ level.

CONCLUSION: With a novel strategy for protein–aptamer interaction, the feasibility of applying QDs‐based fluorescent detection technique to HCV viral protein assay for the development of a protein biochip was demonstrated. This scheme of QDs‐mediated imaging with a target‐oriented specific RNA aptamer for the detection of infectious HCV diseases provides an efficient strategy and a promising new platform for monitoring applications. Copyright © 2010 Society of Chemical Industry