✦ LIBER ✦

Quantitative two-photon Ca2+ imaging via fluorescence lifetime analysis

✍ Scribed by Christian D. Wilms; Hartmut Schmidt; Jens Eilers

Book ID: 113525587
Publisher: Elsevier Science
Year: 2006
Tongue: English
Weight: 369 KB
Volume: 40
Category: Article
ISSN: 0143-4160
DOI: 10.1016/j.ceca.2006.03.006

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## Abstract Two‐photon excitation fluorescence resonance energy transfer (2P‐FRET) imaging microscopy can provide details of specific protein molecule interactions inside living cells. Fluorophore molecules used for 2P‐FRET imaging have characteristic absorption and emission spectra that introduce