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Quantitative thin layer chromatography for the analysis of skin surface lipids

โœ Scribed by Arthur Weissmann


Book ID
104763643
Publisher
Springer-Verlag
Year
1979
Tongue
English
Weight
654 KB
Volume
265
Category
Article
ISSN
0340-3696

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โœฆ Synopsis


Recently a new thin layer chromatography plate (Whatman LK 6 D) became available which is extremely easy to handle and permits highly reproducible qualitative and quantitative analysis. This plate proved to be of great value for the investigation of skin surface lipids. The use of a fatty acid methyl ester as an internal standard makes it unnecessary to employ additional gravimetrical or photometrical methods for quantitative lipid analysis. The method presented in this paper is simpler and requires much less time than alternative procedures and allows a large number of lipid samples to be processed simultaneously.


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