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Quantitative surface studies of protein adsorption by infrared spectroscopy: II. Quantification of adsorbed and bulk proteins

✍ Scribed by David J. Fink; Tim B. Hutson; Krishnan K. Chittur; R.Michael Gendreau


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
707 KB
Volume
165
Category
Article
ISSN
0003-2697

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✦ Synopsis


Attenuated total reflectance Fourier transform infrared spectra of surface-adsorbed proteins are correlated with concentration measurements determined by 125I-labeled proteins. This paper demonstrates that linear correlations between the intensity of the major bands of proteins and the quantity of proteins can be obtained for human albumin and immunoglobulin G up to surface concentrations of approximately 0.25 microgram/cm2. A poorer correlation was observed for human fibrinogen. A linear correlation was also observed between the concentration in the bulk solution and the major bands of albumin up to a concentration of 60 mg/ml.


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