Quantitative studies on colony-forming units in isogenic radiation chimeras
✍ Scribed by Playfair, John H. L. ;Cole, Leonard J.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1965
- Tongue
- English
- Weight
- 662 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0095-9898
No coin nor oath required. For personal study only.
✦ Synopsis
Lethally irradiated LAFl mice were protected by various numbers of isogenic bone marrow cells. At intervals, cells from femoral bone marrow and spleen were assayed for their content of colony forming units (CFU) by retransplantation into other lethally irradiated mice and counting of spleen nodules at eight days. CFU numbers i n femoral marrow were restored to preirradiation levels by about eight weeks. In the spleen, restoration was earlier and more rapid and for several weeks the CFU content exceeded the normal level. Prior splenectomy did not alter the pattern of CFU restoration in the femoral marrow postirradiation. However, splenectomy did appear to slightly improve survival of irradiated marrdw-protected mice. It is argued that the CFU may not be the essential protective cell, and the existence of a precursor "stem" cell i n normal marrow is postulated.