Quantitative studies of a mycobacterial phage — host system I. Characteristic properties of the phage MyF3P/59a
✍ Scribed by Hana Mohelská; Anežka Makovcová
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 937 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Quantitative studies of a mycobacterial phagehost system I. Characteristic properties of the phage MyF, P/59a H ~x -4 MOHELSICL and A N E ~K A MAKOVCOV~ 1968 29-37 (Einyeyanyen am 21. 5. 1967) Since 1947, when GARDNER and WEISER (1947) succeeded in isolating the first mycobacterial phage from soil, quite a lot ofauthors (HAUDUROY and ROSin the study of phages attacking mycobacteria. Only in 1954, FROMAN and BOGEN (1954) were the first to have discovered a phage invading also Mycobacterium horninis and bovis.
The special communications concerning the mycobacteriophage vital manifestations, e . g . phage-host interaction, sensitivity t o different factors of the environment etc., are sporadic. The main reason of this is that very diverse types of phages and host strains are used for experiments, and also because mycobacteria, due t o the character of their growth, are very unsuitable for a number of quantitative phage experiments.
We studied a mycobacterial phage isolated in Czechoslovakia (SULA and S U L O V ~ 1959) t o obtain informations regarding its morphology, sensitivity to UV radiation and temperature and the efficacy of the homologous antiphage sera. SET 1948, P E N S O and ORTALI 1949, WHITTAKER 1950 and others) were engaged
Material and Methods
Bacteriophage
High titer phage stock suspension was prepared according to the method of SWANSTROM and ADAMS (1951) and freeze-dried in 1,5 per cent Ka-glutamate.
Host strain
Mycobacterium fortuitum designated minetti (PENSO and co-workers 1962) was used both for phage reproduction and phage titrations. The strain was obtained from Prof. PEN-