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Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples

✍ Scribed by Erickson, Heidi S; Albert, Paul S; Gillespie, John W; Rodriguez-Canales, Jaime; Marston Linehan, W; Pinto, Peter A; Chuaqui, Rodrigo F; Emmert-Buck, Michael R

Book ID: 109946764
Publisher: Nature Publishing Group
Year: 2009
Tongue: English
Weight: 1021 KB
Volume: 4
Category: Article
ISSN: 1750-2799
DOI: 10.1038/nprot.2009.61

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## Abstract Recent advances in laser microdissection allow for precise removal of pure cell populations from morphologically preserved tissue sections. However, RNA from paraffin‐embedded samples is usually degraded during microdissection. The purpose of this study is to determine the optimal fixat