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Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay

✍ Scribed by Ogrean, Christy; Jackson, Ben; Covino, James


Book ID
120243448
Publisher
MyJoVE Corporation
Year
2010
Tongue
English
Weight
130 KB
Volume
40
Category
Article
ISSN
1940-087X

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Quantitative analysis of cytomegalovirus
✍ Tanaka, Naoko; Kimura, Hiroshi; Iida, Keiji; Saito, Yumiko; Tsuge, Ikuya; Yoshim πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 191 KB

A novel real-time PCR assay system was developed to quantify the cytomegalovirus (CMV) genome load. The real-time PCR assay could detect from 6 to over 10 6 copies of CMV-DNA with a wide linear range. The virus load of immunocompromised patients with symptomatic CMV infections was quantified and com