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Quantitative real-time PCR for detection of neurotoxin genes of Clostridium botulinum types A, B and C in equine samples

✍ Scribed by Johnson, Amy L.; McAdams-Gallagher, Susan C.; Sweeney, Raymond W.

Book ID: 121394511
Publisher: Elsevier Science
Year: 2014
Tongue: English
Weight: 283 KB
Volume: 199
Category: Article
ISSN: 1090-0233
DOI: 10.1016/j.tvjl.2013.10.023

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## Background Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing transcription profiles is accurate normalisation. In most of the studies published on real-time PC