Quantitative methodology for corticosteroids based on chemical oxidation to electrophilic products for electron capture-negative chemical lonization using capillary gas chromatography-mass spectrometry: I. Assessment of feasibility in the analysis of horse urine for dexamethasone
✍ Scribed by G.R. Her; J.T. Watson
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 547 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Sensitive and specific methodology based on capillary column gas chromatography-electron capture-negative chemical ionization-mass spectrometry has been developed for the quantitative analysis of corticosteroids from biological fluids. The feasibility of this method is demonstrated in the quantitative analysis of dexamethasone in horse urine following administration of the drug. A structurally similar compound, 6a-methylprednisolone, is added to the urine as an internal standard. The free dexamethasone and the internal standard are extracted and oxidized to highelectron-athnity 1,4-androskdiene-3,11,1 'I-trione structural analogs and then analyzed by capiIlary column gas chromatography-electron capture-negative chemical ionization-mass spectrometry.