A simple and sensitive method for quantification of sphingosine in cellular lipid extracts was developed. The assay is based on quantitative conversion of sphingosine to \(\mathrm{N}-\left[{ }^{3} \mathrm{H}\right]\) acetylated sphingosine \(\left[\left[{ }^{3} \mathrm{H}\right] \mathrm{C}_{2}{ }^{-
โฆ LIBER โฆ
Quantitative Measurement of Sphingosine 1-Phosphate in Biological Samples by Acylation with Radioactive Acetic Anhydride
โ Scribed by Y. Yatomi; F.Q. Ruan; H. Ohta; R.J. Welch
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 522 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0003-2697
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This paper describes a simultaneous analytical method for the measurement of sphingoid base 1-phosphates and sphingoid bases from a variety of biological samples. This method consists of two steps of sample pretreatment: the enzymatic dephosphorylation of sphingoid base 1-phosphates by alkaline phos