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Quantitative measurement of fathead minnow vitellogenin by liquid chromatography combined with tandem mass spectrometry using a signature peptide of vitellogenin

✍ Scribed by Fagen Zhang; Michael J. Bartels; Julie C. Brodeur; Kent B. Woodburn


Book ID
111847509
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
167 KB
Volume
23
Category
Article
ISSN
0730-7268

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## Abstract In this work, we present a simple method for absolute quantification of plasma vitellogenin from both rainbow trout and Atlantic salmon. Plasma samples obtained from control and β‐estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and an