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Quantitative mass spectrometry-based assay development and validation: From small molecules to proteins

✍ Scribed by Božović, Andrea; Kulasingam, Vathany


Book ID
121324687
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
761 KB
Volume
46
Category
Article
ISSN
0009-9120

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From Small-Molecule Reactions to Protein
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This work introduces stopped-flow electrospray ionization (ESI) mass spectrometry (MS) as a method for studying fast biochemical reaction kinetics. After initiating a reaction by rapid mixing of two solutions, the mixture is transferred to a reaction vessel and a steady liquid flow to the ESI source