Quantitative magnesium analysis of mononuclear cells: Electron probe x-ray microanalysis and atomic absorption spectroscopy methods compared
✍ Scribed by Gregory R. Hook; Jeanette M. Hosseini; Ronald J. Elin
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 754 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
We quantitated magnesium concentration of pooled human peripheral mononuclear blood cell populations and individual human peripheral mononuclear blood cells by flame atomic absorption spectroscopy and electron probe Xray microanalysis, respectively. We found no significant difference ( p > .l) between the mean cell magnesium concentrations (61.4 5 10.9 and 51.2 ? 11.2 mmolikg dry weight) determined by electron probe X-ray microanalysis and flame atomic absorption spectroscopy, re-. . . spectively. Using electron probe X-ray microanalysis, we found a 20% variation in magnesium concentration among individual cells. The cell magnesium concentration was independent of cell size, but the cell magnesium content increased with cell volume. We discuss the analytical limitations and artifacts of flame atomic absorption spectroscopy and electron probe X-ray microanalysis for determination of mononuclear blood cell magnesium.