𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Quantitative Intensity-Based FRET Approaches—A Comparative Snapshot

✍ Scribed by Zeug, André; Woehler, Andrew; Neher, Erwin; Ponimaskin, Evgeni G.

Book ID
117993357
Publisher
Biophysical Society
Year
2012
Tongue
English
Weight
184 KB
Volume
103
Category
Article
ISSN
0006-3495

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## Abstract The intensity‐based ratiometric FRET (fluorescence resonance energy transfer) method is a powerful technique for following molecular interactions in living cells. Since it is not based on irreversibly destroying the donor or the acceptor fluorophores, the time course of changes in FRET