Quantitative in vivo cytokine analysis at synthetic biomaterial implant sites
✍ Scribed by Analiz Rodriguez; Howard Meyerson; James M. Anderson
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 263 KB
- Volume
- 89A
- Category
- Article
- ISSN
- 1549-3296
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
To further elucidate the foreign body reaction, investigation of cytokines at biomaterial implant sites was carried out using a multiplex immunoassay and ELISA. Macrophage activation cytokines (IL‐1β, IL‐6, and TNFα), cytokines important for macrophage fusion (IL‐4 and IL‐13), antiinflammatory cytokines (IL‐10 and TGFβ), chemokines (GRO/KC, MCP‐1), and the T‐cell activation cytokine IL‐2 were quantified at biomaterial implant sites. Empty cages (controls) or cages containing synthetic biomedical polymer (Elasthane 80A (PEU), silicone rubber (SR), or polyethylene terephthalate (PET)) were implanted subcutaneously in Sprague‐Dawley rats for 4, 7, or 14 days, and cytokines in exudate supernatants and macrophage surface adhesion and fusion were quantified. The presence of a polymer implant did not affect the levels of IL‐1β, TGFβ, and MCP‐1 in comparison to the control group. IL‐2 was not virtually detected in any of the samples. Although the levels of IL‐4, IL‐13, IL‐10, and GRO/KC were affected by polymer implantation, but not dependent on a specific polymer, IL‐6 and TNFα were significantly greater in those animals implanted with PEU and SR, materials that do not promote fusion. The results indicate that differential material‐dependent cytokine profiles are produced by surface adherent macrophages and foreign body giant cells in vivo. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009