Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization

✍ Scribed by M. Sato; S. Suzuki; Y. Yasuda; H. Kawauchi; N. Kanno; Y. Sato

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 412 KB
• Volume: 174
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90065-6

✦ Synopsis

A new method for the quantitative analysis of acidic opines-alanopine, strombine, tauropine, and @-alanopine-is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines aher partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, lo-30 pmol minimum detection, and better reproducibility than the highvoltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of @-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.