Quantitative gel-permeation chromatography without standards

✍ Scribed by Robert E. Synovec; Edward S. Yeung

• Book ID: 104144728
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 511 KB
• Volume: 283
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96253-8

✦ Synopsis

Samples of motor oils are studied using gel-permeation chromatography and refractive index detection. When two eluents of similar properties but different refractive indices are used in succession, two distinct chromatograms are obtained for the same analyte. This information can be used to predict the volume fraction, the mole fraction, and the refractive index of each fraction of the analyte as it elutes from the column. The procedure does not involve complete separation of the components, identification of the components, or knowing the physical properties of the components of the analyte.