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Quantitative evaluation of thin-layer chromatograms. 3. The calculation of fluorescence using multilayer models

✍ Scribed by Prošek, M. ;Medja, A. ;Kučan, E. ;Katič, M. ;Bano, M.

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
402 KB
Volume
3
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Changes of fluorescence intensity at the far side (transmission) and near side (remission) in cases where a spot of a fluorogen lies in different sublayers are calculated using a multilayer model. Examples shown are: the relative intensity of fluorescence in the layer as a function of the absorption coefficient of a sorbent at the excitation wavelength; the relative intensity of fluorescence at the far and near sides of a TLC plate; the influence of using too broad a monochromatic filter at the emission side on the results of fluorescence measurements. Advantages of near‐side scanning of fluorescence compared to far‐side scanning are discussed. The most important source of errors in fluorodensitometric measurements is the effect of secondary chromatography.

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