Quantitative evaluation of periprosthetic infection by real-time polymerase chain reaction: a comparison with conventional methods
โ Scribed by Yushi Miyamae; Yutaka Inaba; Naomi Kobayashi; Hyonmin Choe; Hiroyuki Ike; Takako Momose; Shusuke Fujiwara; Tomoyuki Saito
- Book ID
- 119227187
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 430 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0732-8893
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Four quantitative reverse transcription-PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and decay. Mouse fibroblasts (NIH 3T3) transfected with the human โค-globin open reading frame/c-myc 3-untranslated region chimeric gene under control of the c-fos promoter (fosglo
Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference