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Quantitative evaluation of fluorescence in situ hybridization (FISH) signals in uncultured coelomic cells

✍ Scribed by D. G. Crüger; G. Bruun-Petersen; S. Kølvraa

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
278 KB
Volume
17
Category
Article
ISSN
0197-3851

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✦ Synopsis

Uncultured coelomic cells were hybridized with alpha satellite DNA probes representing chromosomes X, Y, 18, and 13/21 in order to evaluate the distribution of hybridization signals obtained by fluorescence in situ hybridization (FISH) analysis of this cell type. Cells from 26 samples were hybridized with the X probe and the Y probe was hybridized with cells from 25 of the samples. Cells from 16 and 11 samples were hybridized with an 18 alpha satellite DNA probe and 13/21 alpha satellite probe, respectively. The evaluation demonstrated that FISH with X, Y, 18, and 13/21 alpha satellite DNA probes on uncultured coelomic cells is a reliable technique since the distribution of hybridization signals is comparable to that seen in uncultured amniotic fluid cells.

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