Quantitative evaluation of chromatographic analysis of complex mixtures by establishing limits of determination

✍ Scribed by L.J. Nagels; W.L. Creten

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 621 KB
• Volume: 169
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86232-7

✦ Synopsis

A method is presented for the calculation of limits of determination in the chromatography of complex samples. This limit, DLS,W, is defined as the minimum concentration (rg g-') of the compound in the original sample that should be present in an extract taken at random from a well-defined set of extracts, so as to give a probability w that the result will have a relative error smaller than e. The interference caused by other components of the samples is taken into account. This limit of determination was calculated for the determination of quercetin and caffeic acid in European plant extracts by reversed-phase h.p.1.c. with u.v./visible detection. These determination limits were calculated as a function of the detector wavelength (250-400 nm) with a minicomputer. One of the main parameters used in these calculations for optimizing wavelengths of measurement in the chromatography of complex mixtures is a peak density function for the whole chromatogram.