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Quantitative enzymatic synthesis of 2-acetamido-2-deoxy-α-d-glucopyranosyl-1,6-diphosphate

✍ Scribed by Pi-Wan Cheng; Don M. Carlson

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
427 KB
Volume
85
Category
Article
ISSN
0003-2697

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✦ Synopsis

Phosphoacetylglucosamine mutase (EC 2.7.5.2) catalyzes the interconversion of N-acetylglucosamine-l-phosphate and of N-acetylglucosamine-6-phosphate. Glucose-1,6-diphosphate activates this mutase through the formation of Nacetylglucosamine-1 ,ddiphosphate and glucose&phosphate.

By adding glucose-6-phosphate dehydrogenase and an excess of N-acetylglucosamine-&phosphate the reaction is pulled to completion with stoichiometric formation of N-acetylglucosamine-1,6-diphosphate with respect to the amount of glucose-1,6-diphosphate present. The isolation, chemical analysis, molar rotation, and cofactor activity of N-acetylglucosamine-1,6-diphosphate are described.

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