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Quantitative dot-blot assay for proteins using enhanced chemiluminescence

โœ Scribed by E. Heinicke; U. Kumar; D.G. Munoz


Book ID
115972953
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
611 KB
Volume
152
Category
Article
ISSN
0022-1759

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A method for the detection and quantitation of picomole amounts of heparin-binding proteins is described. Proteins are first spotted on nitrocellulose and then incubated with "51-heparin. Binding of heparin to the proteins is detected by radioautography and quantitated by scanning densitometry; pro