A dot-blot assay for heparin-binding pro
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Nobuyoshi Hirose; Michele Krivanek; Richard L. Jackson; Alan D. Cardin
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Article
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1986
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Elsevier Science
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English
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A method for the detection and quantitation of picomole amounts of heparin-binding proteins is described. Proteins are first spotted on nitrocellulose and then incubated with "51-heparin. Binding of heparin to the proteins is detected by radioautography and quantitated by scanning densitometry; pro