Quantitative determination of the stabilizers octanoic acid and N-acetyl-dl-tryptophan in human albumin products
✍ Scribed by M. W. Yu; J. S. Finlayson
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 636 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
Methods were developed for the determination of octanoic acid and N-acetyl-DL-tryptophan, which are used as stabilizers in the human blood-derived therapeutic products normal serum albumin and plasma protein fraction. The method for octanoic acid uses GC; quantitation is achieved using heptanoic acid as the internal standard. The method for N-acetyl-DL-tryptophan is based on UV spectrophotometry of the acid-soluble fraction remaining after precipitation of the protein (€280 for N-acetyl-DL-tryptophan, 5250). The coefficient of variation for replicate determinations of octanoic acid averaged 3.9% (range 2.1-5.576); that of N-acetyl-DL-tryptophan averaged 1.9% (range 0.5-4.0%). Use of these methods for the analysis of 138 lots of commercial products for octanoic acid and 159 lots for N-acetyl-DL-tryptophan showed that the stabilizer contents of 132 and 158 of these lots, respectively, were within 20% of the value indicated on the product label.
Keyphrases 0 N-Acetyl-DL-tryptophan-quantitative determination of stabilizers, octanoic acid, human albumin products 0 Octanoic acid-quantitative determination of stabilizers, N-acetyl-DL-tryptophan, human albumin products 0 Human albumin products-quantitation of stabilizers, octanoic acid and N-acetyl-DL-tryptophan ~ ~~~