## Abstract Fluorescence of acridine orange bound to RNA or DNA in the singleβstranded form including singleβstranded synthetic polyriboβ or polydeoxyribonucleotides was measured in the expectation that some distinct structural characteristic between singleβstranded RNA and DNA might be reflected b
β¦ LIBER β¦
Quantitative determination of single-stranded sections in DNA using the fluorescent probe acridine orange
β Scribed by Sachiko Ichimura; Mitsuo Zama; Hitoshi Fujita
- Book ID
- 115744519
- Publisher
- Elsevier Science
- Year
- 1971
- Weight
- 535 KB
- Volume
- 240
- Category
- Article
- ISSN
- 0005-2787
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Various assays are available for quantification of DNA in solution, but none has been described that is both sensitive and specific for double-stranded (ds) DNA and features practical properties such as low dye and equipment costs, speed, and highly parallel microplate formats. Here we show that qua