Quantitative determination of SC-68328 in dog plasma using flow injection and tandem mass spectrometry

✍ Scribed by Zhang, Ji Y.; Fast, Douglas M.; Schoenhard, Grant L.; Arora, Vinod K.; Belas, Frank J.; Blair, Ian A.

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 121 KB
• Volume: 35
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(200003)35:3<354::aid-jms942>3.0.co;2-8

✦ Synopsis

A flow injection/tandem mass spectrometric assay was developed to quantitate SC-68328 in dog plasma using its stable isotopic analog [ 13 C 4 ]SC-68328 as an internal standard (IS). Since SC-68328, a manganese-based superoxide dismutase mimetic, is very unstable, very polar and adheres to silica-based high-performance liquid chromatographic columns, the analyte and IS were derivatized to their bis-isothiocyanate forms followed by a liquid-liquid extraction with methylene chloride and analyzed using positive ion electrospray mass spectrometric detection. SC-68328 was quantitated using the peak-height ratio of SC-68328 to its IS using MS/MS in the multiple reaction monitoring mode. The lower limit of quantitation of the assay was 0.25 µg ml -1 SC-68328 in dog plasma with an inter-day precision of 11.8% and an accuracy of 113% (n = 12). Acceptable precision and accuracy were also obtained for concentrations in the calibration curve range (0.25-10 µg ml -1 SC-68328 in dog plasma).