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Quantitative determination of prostaglandins A, B and E in the sub-nanogram range

✍ Scribed by Brian J. Sweetman; Jürgen C. Frölich; J.Throck Watson

Book ID
119047822
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
717 KB
Volume
3
Category
Article
ISSN
0090-6980

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A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin.