Quantitative determination of low levels of daunomycin and daunomycinol in plasma by high-performance liquid chromatography

✍ Scribed by R. Hulhoven; J.P. Desager

• Book ID: 104144399
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 387 KB
• Volume: 125
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)93832-9

✦ Synopsis

A high-performance Iiqtid chromato_@-aphic method for the measurement of daunomycin and its main metabolite, daunomycinol, at iow concentrations in plasma is described.

Quantitative determinations were obtained by the use of atiarnycin, another CyStOStatic a&XaCycfine antibiotic, as an internal standard. The SepaIX&OQS were carried out on a 5+-x1 silica microsphere cofumn with a quaternary solvent mixture as etuenti The components in the eluted peaks were detected by visible absorption at 490 nm and there were no interfering peaks. The advantages of the method are spe-c&z&y, sensitivity, minimal pre-ana@sis sample work-lip and small sample Size. The method is Sensitive for plasma fevels of daunomycin and daunomycinot above LO cg/d-Experimental animal data are presented to illustrate the application of the method.