Quantitative correlation between radiation-induced mutagenesis in endogenous genes and transgenes of mouse spermatogonial stem cells
✍ Scribed by H. J. Martus; M. Novak; D. Blecher; A. van Duyn–Goedhart; W. Suter; J. A. Gossen; P. P. W. van Buul
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 64 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
In order to evaluate the pUR288-plasmid transgenic mouse model, utilizing the bacterial lacZ gene as the mutational target, radiation-induced mutagenesis was primarily analyzed in spermatogonial stem cells. A combined hydroxyurea (HU)-X-ray treatment protocol was used, known to sensitize dramatically the induction of mutations in endogenous genes. In the testes of untreated animals, a mutant frequency of 6.7 Ϯ 4.4 ϫ 10 -5 was found. In animals treated with HU or X ray alone, moderate elevations were seen (factors of about 4 and 2 over untreated animal values). In testes of mice having received the HU ϩ X-ray combination treatment, a mutant frequency of 63.0 Ϯ 36.1 ϫ 10 -5 was found. The results obtained showed a good quantitative correlation between endogenous genes and the transgene, indicating the suitability of pUR288 transgenic mice for also efficiently recording radiation-induced genetic damage. Radiosensitization, seen in spermatogonial stem cells, was not observed in other studied organs such as spleen, brain, or lung.