Quantitative chromatographic analysis of toxins in single mushrooms of amanita phalloides

✍ Scribed by Heinz Faulstich; Dionysios Georgopoulos; Maria Bloching

• Book ID: 104145906
• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 643 KB
• Volume: 79
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85296-1

✦ Synopsis

By a simple combination of column and thin-layer chromatography, all of the toxic compounds in I-Z g of air-dried mushrooms of Amanita ~kalloides (25 g of fresh material) were isolated and quantitatively determined.

In pure, non-resolved, fractions of toxins, e.g., mixtures of phalloin, phalloidin and phallisin, the amount of each tosin was determined after acid hydrolysis of the peptides by ion-exchange chromatography of the corresponding y-hydrosyleucine and y-hydroxyisoleucine lactones, which in ion-eschange amino acid analysis behave like basic amino acids. For y-lactones with chiral y-carbon atoms, epimerisation during hydrolysis was observed. By this direct analysis of crude extracts, it was observed that the portion of acidic toxins is much larger than has been determined so far, namely up to 70% of all toxic peptides. It was further discovered that phallacidin is accompanied by an acidic, higher hydroxylated analogue. It contains, like phallisin, y,&rS'-trihydroxyleucine and will be called phallisacin.

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